| Gene name: | MDM2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Adipose stromal cell |
| Experiment: | SA-β-gal activity assay//qRT-PCR//Cell morphological analysis |
| Description: | Furthermore, as expected, antagonizing MDM2 decreased the expression of HMGA2 significantly supporting the assumption that the p14Arfrepression of HMGA2 is TP53-dependent.Thus, antagonizing MDM2 can induce senescence in mesenchyme-derived stem cells. The reduced levels of HMGA2 expression are also accompanied by an increased number of β-gal positive cells. For these experiments, canine ADSCs were treated with 30 lM and 50 lM nutlin-3, respectively, for 72 hr. The number of β-gal positive cells was then determined by counting 500–600 cells. With both concentrations,the percentage of positive cells was drastically increased (30 lM: 54.0%; 50 lM: 60.0%) compared with the control cells (9.9%). Also, the increased percentage of positive cells was accompanied by clear morphological changes leading to a majority of flat enlarged cells with an absence of mitoses. |
| Target gene: | HMGA2 |
| Official symbol(s): | HMGA2 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | Furthermore, as expected, antagonizing MDM2 decreased the expression of HMGA2 significantly. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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