| Gene name: | RB1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Knockdown |
| Description: | We observed signs of senescence in shR1 as detected by in situ senescence-associated-beta-galactosidase assay, compared with cells transduced with controls shRNAs.Beta-galactosidasepositive cells showed the characteristic senescent morphology (flat, enlarged cells). Moreover, we detected an increase in the percentage of bi-nucleate cells in shR1 (data not shown), which is another hallmark of senescent cells. |
| Target gene: | P53//P27//P21 |
| Official symbol(s): | P53//P27//P21 |
| R-AG-Target gene: | --//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | We detected a significant up-regulation (p < 0.05) of p53 protein in shR1 along with an increase in the level of p27kip1 and p21cip1, whereas no modification was observed in p16Ink4a. the increased expression of these genes was also observed at mRNA level. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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