AgingReG
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Aging Overview

Aging gene

Gene name: NDRG1
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: HepG2
Gene ID: 10397
Category: protein coding
Phenotype: Hepatocellular carcinoma
Experimental category: HL
PMID: 24302615
Experiment: SA-β-gal activity assay//Knockdown
Description: A large percentage (about 80%) of HepG2 cells transfected with NDRG1-specific siRNA was positive for SA-β-Gal staining.

Regulatory relationship

Regulatory pathway: GSK3β-P53/-P16
R-AG-Pathway: --
Official symbol(s): GSK3B-TP53-CDKN2A
Pathway experiment: SA-β-gal activity assay//Western blot
Pathway description: The percentage of SA-β-Gal positive cells was also reduced in HepG2 cells co-transfected with NDRG1-specific siRNA and siRNA against p53, p16, p53 & p16, or GSK-3. Western blot results indicated that co-transfection of NDRG1-specific siRNA and siRNA against p53, p16, or p53 & p16 increased p-Rb level which was decreased by NDRG1-specific siRNA . Additionally, GSK-3β siRNA decreased GSK-3β, GSK-3β 9ser, p53, p21 and p16 levels, indicating that GSK-3β can stabilize p53 and p16 levels in HepG2 cells with NDRG1 suppression. Consistently, a decrease in p21 (a p53 downstream target) and increased p-Rb were detected in cells co-treated with NDRG1-siRNA and PFT-α.

Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.


Pathway view

AKT phosphorylates targets in the cytosolAKT(PKB)-Bad signaling ( EPO signaling pathway(JAK2 STAT1 STAT3 STAT5) ) AKT(PKB)-Bad signaling ( IL-7 signaling(JAK1 JAK3 STAT5) ) AKT(PKB)-GSK3beta signaling ( Insulin receptor signaling (D. melanogaster) ) AKT(PKB)-GSK3beta signaling ( Insulin receptor signaling (Mammal) ) Alzheimer disease-presenilin pathwayAndrogenReceptorAngiogenesisAPC truncation mutants have impaired AXIN bindingAurora A signalingAXIN missense mutants destabilize the destruction complexB-WICH complex positively regulates rRNA expressionBCRBeta-catenin phosphorylation cascadeBeta catenin degradation signaling (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) Beta catenin degradation signaling (Canonical) ( Canonical Wnt signaling pathway Diagram ) Beta catenin degradation signaling (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) Beta catenin degradation signaling (Canonical) ( Mammalian Wnt signaling pathway Diagram ) Beta catenin degradation signaling (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) Beta catenin degradation signaling (Mammal) ( Mammalian Wnt signaling pathway Diagram ) BMP receptor signalingC-MYC pathwayCadherin signaling pathwayCanonical Wnt signaling pathwayCDC42 signaling eventsCi/Gli processing signaling ( Hedgehog signaling pathway ) Class I PI3K signaling events mediated by AktConstitutive Signaling by AKT1 E17K in CancerCRMPs in Sema3A signalingDegradation of beta-catenin by the destruction complexDegradation of beta cateninDegradation of GLI2 by the proteasomeDisassembly of the destruction complex and recruitment of AXIN to the membraneDrosophila Toll-like receptor signaling ( Drosophila Toll-like receptor signaling ) EGFR1Gli processing signaling ( Hedgehog signaling pathway ) GLI3 is processed to GLI3R by the proteasomeGlucocorticoid receptor regulatory networkHedgehog signaling events mediated by Gli proteinsHedgehog signaling pathwayHeterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathwayIL-7IL1IL3IL5IL6Insulin-mediated glucose transportInsulin receptor signaling ( Insulin receptor signaling (D. melanogaster) ) Insulin receptor signaling (Mammal) ( Insulin receptor signaling (Mammal) ) Insulin/IGF pathway-protein kinase B signaling cascadeIntegrin-linked kinase signalingInterleukin signaling pathwayKitReceptorLeptinLKB1 signaling eventsLPA receptor mediated eventsMisspliced GSK3beta mutants stabilize beta-cateninNotchp53 pathwayPDGF signaling pathwayPI3 kinase pathwayPositive regulation of (Transcription of SOCS by STAT dimer) in JAK STAT pathway ( JAK-STAT pathway and regulation pathway Diagram ) Presenilin action in Notch and Wnt signalingProlactinRas PathwayReelin signaling pathwayRegulation of Androgen receptor activityRegulation of HSF1-mediated heat shock responseRole of Calcineurin-dependent NFAT signaling in lymphocytesS33 mutants of beta-catenin aren't phosphorylatedS37 mutants of beta-catenin aren't phosphorylatedS45 mutants of beta-catenin aren't phosphorylatedSignaling events mediated by Stem cell factor receptor (c-Kit)Stabilization and accumulation of cytoplasmic beta-catenin (Canonical) ( C. elegans endoderm induction Wnt signaling pathway Diagram ) Stabilization and accumulation of cytoplasmic beta-catenin (Canonical) ( Canonical Wnt signaling pathway Diagram ) Stabilization and accumulation of cytoplasmic beta-catenin (Canonical) ( Drosophila Wingless/Wnt signaling pathway Diagram ) Stabilization and accumulation of cytoplasmic beta-catenin (Canonical) ( Mammalian Wnt signaling pathway Diagram ) Stabilization and accumulation of cytoplasmic beta-catenin (Canonical) ( Xenopus axis formation Wnt signaling pathway Diagram ) Stabilization and accumulation of cytoplasmic beta-catenin (Mammal) ( Mammalian Wnt signaling pathway Diagram ) T41 mutants of beta-catenin aren't phosphorylatedTCRTGF_beta_ReceptorTrk receptor signaling mediated by PI3K and PLC-gammaTruncations of AMER1 destabilize the destruction complexTWEAKValidated transcriptional targets of deltaNp63 isoformsVEGF signaling pathway ( VEGF signaling pathway ) WntWnt signaling pathwayErbB signaling pathwayChemokine signaling pathwayCell cycleWnt signaling pathwayHedgehog signaling pathwayAxon guidanceFocal adhesionT cell receptor signaling pathwayB cell receptor signaling pathwayCircadian rhythm - flyNeurotrophin signaling pathwayInsulin signaling pathwayMelanogenesisAlzheimer's diseaseHepatitis CPathways in cancerColorectal cancerEndometrial cancerProstate cancerBasal cell carcinomaHs_Cell_Cycle_WP179_89516Hs_ErbB_Signaling_Pathway_WP673_91869Hs_Corticotropin-releasing_hormone_signaling_pathway_WP2355_90017Hs_Association_Between_Physico-Chemical_Features_and_Toxicity_Associated_Pathways_WP3680_90113Hs_Focal_Adhesion_WP306_80308Hs_Factors_and_pathways_affecting_insulin-like_growth_factor_(IGF1)-Akt_signaling_WP3850_88165Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193Hs_Wnt_Signaling_Pathway_Netpath_WP363_78571Hs_T-Cell_antigen_Receptor_(TCR)_pathway_during_Staphylococcus_aureus_infection_WP3863_90702Hs_Alzheimers_Disease_WP2059_87372Hs_Copper_homeostasis_WP3286_89205Hs_VEGFA-VEGFR2_Signaling_Pathway_WP3888_90000Hs_Selenium_Metabolism_and_Selenoproteins_WP28_71888Hs_Neural_Crest_Differentiation_WP2064_79263Hs_TNF_related_weak_inducer_of_apoptosis_(TWEAK)_Signaling_Pathway_WP2036_89910Hs_Cardiac_Hypertrophic_Response_WP2795_85087Hs_MicroRNAs_in_cardiomyocyte_hypertrophy_WP1544_89794Hs_IL17_signaling_pathway_WP2112_89838Hs_Regulation_of_Microtubule_Cytoskeleton_WP2038_90889Hs_IL-5_Signaling_Pathway_WP127_78498Hs_Focal_Adhesion-PI3K-Akt-mTOR-signaling_pathway_WP3932_91101Hs_IL-6_signaling_pathway_WP364_89832Hs_Prolactin_Signaling_Pathway_WP2037_90015Hs_Brain-Derived_Neurotrophic_Factor_(BDNF)_signaling_pathway_WP2380_89803Hs_IL-7_Signaling_Pathway_WP205_89854Hs_B_Cell_Receptor_Signaling_Pathway_WP23_89900
Activation of PUMA and translocation to mitochondriaAP-1 transcription factor networkApoptosis signaling pathwayAssociation of TriC/CCT with target proteins during biosynthesisAurora A signalingAutodegradation of the E3 ubiquitin ligase COP1BARD1 signaling eventsDirect p53 effectorsDNA Damage/Telomere Stress Induced SenescenceEGFR1Factors involved in megakaryocyte development and platelet productionFormation of Senescence-Associated Heterochromatin Foci (SAHF)G2/M CheckpointsG2/M DNA damage checkpointGlucocorticoid receptor regulatory networkHuntington diseaseHypoxic and oxygen homeostasis regulation of HIF-1-alphaLKB1 signaling eventsOncogene Induced SenescenceOxidative Stress Induced Senescencep53 pathway by glucose deprivationp53 pathwayp53 pathwayP53 pathway feedback loops 1p53 pathway feedback loops 2p75(NTR)-mediated signalingPLK3 signaling eventsPre-NOTCH Transcription and TranslationRecruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaksRegulation of TP53 Activity through AcetylationRegulation of TP53 Activity through Association with Co-factorsRegulation of TP53 Activity through MethylationRegulation of TP53 Activity through PhosphorylationRegulation of TP53 DegradationSignaling events mediated by HDAC Class IIISignaling mediated by p38-alpha and p38-betaStabilization of p53SUMOylation of transcription factorsTGF_beta_ReceptorThe role of GTSE1 in G2/M progression after G2 checkpointTNFalphaTP53 Regulates Metabolic GenesTP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertainTP53 Regulates Transcription of Caspase Activators and CaspasesTP53 Regulates Transcription of Death Receptors and LigandsTP53 Regulates Transcription of DNA Repair GenesTP53 Regulates Transcription of Genes Involved in Cytochrome C ReleaseTP53 Regulates Transcription of Genes Involved in G1 Cell Cycle ArrestTP53 Regulates Transcription of Genes Involved in G2 Cell Cycle ArrestTP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertainValidated targets of C-MYC transcriptional activationVinblastine Action PathwayVincristine Action PathwayVindesine Action PathwayVinorelbine Action PathwayMAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayAmyotrophic lateral sclerosis (ALS)Huntington's diseaseHepatitis CPathways in cancerColorectal cancerPancreatic cancerEndometrial cancerGliomaProstate cancerThyroid cancerBasal cell carcinomaMelanomaBladder cancerChronic myeloid leukemiaSmall cell lung cancerNon-small cell lung cancerHs_Cell_Cycle_WP179_89516Hs_Tryptophan_metabolism_WP465_85055Hs_ErbB_Signaling_Pathway_WP673_91869Hs_DNA_Damage_Response_(only_ATM_dependent)_WP710_79974Hs_TP53_Network_WP1742_71700Hs_DNA_Damage_Response_WP707_82937Hs_miRNA_Regulation_of_DNA_Damage_Response_WP1530_84694Hs_Nuclear_Receptors_in_Lipid_Metabolism_and_Toxicity_WP299_89331Hs_Gastric_Cancer_Network_2_WP2363_87523Hs_Hepatitis_C_and_Hepatocellular_Carcinoma_WP3646_88640Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193Hs_Integrated_Breast_Cancer_Pathway_WP1984_82941Hs_Apoptosis_Modulation_and_Signaling_WP1772_91293Hs_Alzheimers_Disease_WP2059_87372Hs_Copper_homeostasis_WP3286_89205Hs_Integrated_Cancer_Pathway_WP1971_82939Hs_ATM_Signaling_Pathway_WP2516_90247Hs_DNA_IR-Double_Strand_Breaks_(DSBs)_and_cellular_response_via_ATM_WP3959_91367Hs_Pathways_Affected_in_Adenoid_Cystic_Carcinoma_WP3651_89271Hs_LncRNA-mediated_mechanisms_of_therapeutic_resistance_WP3672_87395Hs_Bladder_Cancer_WP2828_89143Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520Hs_Steroid_Biosynthesis_WP496_69016Hs_Retinoblastoma_(RB)_in_Cancer_WP2446_87639Hs_G1_to_S_cell_cycle_control_WP45_80001
AP-1 transcription factor networkC-MYB transcription factor networkC-MYC pathwayCi/Gli processing signaling ( Hedgehog signaling pathway ) Coregulation of Androgen receptor activityCyclin D associated events in G1E2F transcription factor networkFOXM1 transcription factor networkGli processing signaling ( Hedgehog signaling pathway ) Hypoxic and oxygen homeostasis regulation of HIF-1-alphaOncogene Induced SenescenceOxidative Stress Induced Senescencep53 pathwayRegulation of nuclear beta catenin signaling and target gene transcriptionRegulation of retinoblastoma proteinRegulation of TP53 DegradationSenescence-Associated Secretory Phenotype (SASP)SUMOylation of DNA damage response and repair proteinsSUMOylation of transcription factorsValidated transcriptional targets of AP1 family members Fra1 and Fra2Validated transcriptional targets of deltaNp63 isoformsValidated transcriptional targets of TAp63 isoformsCell cyclep53 signaling pathwayPathways in cancerPancreatic cancerGliomaMelanomaBladder cancerChronic myeloid leukemiaNon-small cell lung cancerHs_Cell_Cycle_WP179_89516Hs_TP53_Network_WP1742_71700Hs_Apoptosis_WP254_88977Hs_Senescence_and_Autophagy_in_Cancer_WP615_81193Hs_Bladder_Cancer_WP2828_89143Hs_Signaling_Pathways_in_Glioblastoma_WP2261_89520
About risk SNP and eQTL

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Data source of:
Expression of gene(s)
Cell line (ENCODE) Primary cell (ENCODE) Normal tissue (GTEx) Cell line (CCLE)