| Gene name: | TP53 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PCI-13 |
| Gene ID: | 7157 |
| Category: | protein coding |
| Phenotype: | Squamous cell carcinoma |
| Experimental category: | L |
| PMID: | 25766317 |
| Experiment: | Colony formation assay//SA-β-gal activity assay |
| Description: | Clonogenic assays showed that the 4-Gy surviving fraction in TP53 null or mutant cells was significantly higher than in cells with wtp53. Being that senescence is the primary mechanism of radiation-induced cell death in HNSCC cells, a senescence-associated β-galactosidase (SA-β-gal) staining was performed and showed that 4 days after treatment with 4-Gy ionizing radiation, cells with wtp53 had a significantly higher SA-β-gal-positive staining compared with the SA-β-galpositive staining in TP53 null and all TP53 mutant cells. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot |
| Target gene description: | Among the mutp53 bearing cells, only PCI-13 A161S had minimal, short-lived expression of the p21 gene compared with the enhanced, long-term expression seen in wtp53 cells. All other mutp53 or TP53 null cell lines had no significant elevation in p21 mRNA. Western blot analysis demonstrated long-term expression of the p21 protein in wtp53 cells, with no expression in mutp53 and TP53 null cells, but short-term expression in PCI-13 A161S cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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