| Gene name: | CARM1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Human bronchial epithelial cell |
| Gene ID: | 10498 |
| Category: | protein coding |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experimental category: | HL |
| PMID: | 31461302 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | There was a significant increase in the number of beta-galactosidase positive cells in the airways of NA-treated Carm1+/- mice compared to NA-treated WT animals (31.8 ± 3.4% vs 8.2 ± 2.8%, p<0.001). Consistent with mouse airway epithelial cells, the siCARM1-transfected 337 human cells showed a significantly higher percentage of beta-galact osidase-positive cells compared with 338 scrambled controls (13.2 ± 0.6% vs 3.7 ± 0.6%, p<0.001). |
| Target gene: | SIRT1//P53 |
| Official symbol(s): | SIRT1//P53 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | Moreover, western blot analysis of whole lung homogenates from d14 mice treated with NA revealed a clear reduction in the level of SIRT1 protein in Carm1+/- mice compared to WT controls, with a concomitant increase in p53 levels .Mechanistically, CARM1-silenced cells demonstrated reduced expression of SIRT1, and treating CARM1-silenced cells with the SIRT1 activator resveratrol, reversed the impaired wound healing. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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