| Gene name: | MAP2K4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | As expected, MKK4-KO MEFs, in which the non-canonical NFκB pathway is inactivated, showed more prominent β-galactosidase activity than MKK4-WT when tested in a senescence-associated β-gal activity assay, indicating that MKK4 depletion increased cellular senescence. |
| Target gene: | P16//P21//P27 |
| Official symbol(s): | P16//P21//P27 |
| R-AG-Target gene: | --//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | MKK4 depletion enhanced the protein levels of cyclin-dependent kinase (CDK) inhibitors, p16, p21, and p27, which in turn induced cell growth retardation. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | Western blot |
| Pathway description: | To investigate the direct roles of the non-canonical NFκB pathway, we knocked down NFκB2 using shRNAs in MKK4-WT MEFs.As with MKK4 depletion, NFκB2 knock-down also suppressed cell growth. |
Annotation:
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