| Gene name: | HRAS |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | TIG-3,REF52 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | H‐RasV12‐infected cells displayed a flat, enlarged morphology and had arrested growth by 7?days after infection, which was accompanied by an increase in the activity of a senescence-associated β-galactosidase.?Furthermore, oncogenic Ras elevated the expression of senescence‐related cell cycle regulatory proteins: p53, p21 and p19Arf in REF52 cells and p53, p21 and p16Ink4a in TIG-3 cells. |
| Target gene: | NOX1//NOX4 |
| Official symbol(s): | NOX1//NOX4 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//SA-β-gal activity assay |
| Target gene description: | Nox1 siRNA and Nox4 siRNA consistently abrogated both the accumulation of SA‐β‐gal and up‐regulation of p53, p21 or p16Ink4a in H‐RasV12‐transduced cells. |
| Regulatory pathway: | P53-P21//P16 |
| R-AG-Pathway: | Upregulation//Upregulation |
| Official symbol(s): | TP53-CDKN1A//CDKN2A |
| Pathway experiment: | Knockdown//Western blot//SA-β-gal activity assay |
| Pathway description: | Nox1 siRNA and Nox4 siRNA consistently abrogated both the accumulation of SA‐β‐gal and up‐regulation of p53, p21 or p16Ink4a in H‐RasV12‐transduced cells. |
Annotation:
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