| Gene name: | RBP2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SMMC-7721,HepG2 |
| Gene ID: | 5948 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 23922798 |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | Cell senescence was induced with RBP2 siRNA knockdown for 72 hr in cancer cells. |
| Target gene: | CDKISP16//P21CIP2//P27//HSA-MIR-212 |
| Official symbol(s): | CDKISP16//P21CIP2//P27//HSA-MIR-212 |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot//CHIP//Luciferase reporter assay//qRT-PCR |
| Target gene description: | The expression of known targets of RBP2, namely, the CDKIs p16ink4a, p21CIP2 and p27kip1, was markedly increased with RBP2 siRNA knockdown.siRNA knockdown of RBP2 expression greatly increased luciferase activity of p21CIP2 and slightly but significantly increased activity of p27kip1 in HepG-2 cells; ChIP assay confirmed the results.with RBP2 overexpression, the expression of p21CIP2 and p27kip1 was relatively low .RBP2 expression was markedly repressed with hsa-miR-212 overexpression. To further confirm that RBP2 was negatively regulated by hsa-miR-212, transfection of hsa-miR-212 inhibitor plasmid in the 2 cell lines decreased the expression of hsa-miR-212 and increased that of RBP2. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...