| Gene name: | ID1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | NPTX |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | The AS Id-1 clones displayed several morphological changes compared with the parental NPTX cells, characterized by enlarged, vacuolized and flattened cell bodies and loss of light refractability.The SA-β-gal staining further confirmed that AS Id-1 clones contained significantly more senescent cells .In addition, inactivation of Id-1 resulted in a decrease in S-phase cells , and an increase in G2/M phase cells. |
| Target gene: | P21/WAF1//P27 |
| Official symbol(s): | P21/WAF1//P27 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//RT-PCR |
| Target gene description: | Compared with the pBabe control, AS Id‐1 clones had much lower Id‐1 expression, along with increased p21WAF1/Cip1 and p27KIP1 protein levels.In agreement with the Western‐blot results, the addition of TGF‐β1 had a positive effect on p21WAF1/Cip1 and p27KIP1 mRNA expression and a negative effect on Id‐1 mRNA expression in AS Id‐1 clones. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...