| Gene name: | SP1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | Knockdown//SA-β-gal activity assay |
| Description: | The results showed that Sp1-overexpressed cells were strongly stained blue versus the control. However, there were only a few dispersed cells that were SA-β-Gal-stained in the Sp1 knocked-down cells. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Western blot |
| Target gene description: | As determined by luciferase activity, Sp1 activated the p16INK4a promoter in a dose-dependent manner in both young and senescent 2BS cells,the p16INK4a promoter activity was inhibited by MTR in a dose-dependent manner in both young and senescent 2BS cells. Further, the p16INK4a expression was also reduced at mRNA and protein levels in 2BS cells, by 66% (mRNA level) and 48% (protein level) respectively, in the senescence group with 24 hours of MTR treatment.Western Blot showed that si-Sp1 remarkably reduced the expression of Sp1, which in turn lead to a reduction of p16INK4a expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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