| Gene name: | IGFBPL1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HEC-1A |
| Gene ID: | 347252 |
| Category: | protein coding |
| Phenotype: | Endometrial cancer |
| Experimental category: | L |
| PMID: | 29067463 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | SA-β-galactosidase staining, a golden standard for cellular senescence, indicated that HEC-1A-IGFBP-rP1 cells had a higher proportion of SA-β-galactosidase positive cells than the control cells. On the contrary, SA-β-galactosidase activity significantly decreased in Ishikawa-siRNA (HEC-1A-IGFBP-rP1) cells compared with negative controls . Moreover, p-RB, a key regulator in cellular senescence, was significantly reduced in HEC-1A-IGFBP-rP1 cells than control cells while other senescence-related proteins p21. p53 and p16, increased ~four-fold. 55-fold and 24-fol in HEC-1A-IGFBP-rP1, respectively. In contrast, Ishikawa-siRNA (IGFBP-rP1) cells presented a 2.8-fold increased expression of p-RB (P<0.01) and a decrease level of p16, p21 and p53 protein than control cells. |
| Regulatory pathway: | ERK |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | MAPK1 |
| Pathway experiment: | SA-β-gal activity assay |
| Pathway description: | HEC-1A-IGFBP-rP1 cells with PD98059 presented more SA-β-galactosidase positive cells than HEC-1A with PD98059 treatment. |
Annotation:
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