| Gene name: | EZH2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MKN28,SGC-7901 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | In MKN28 and SGC7901 cells, a series of phenotypic changes which are the hallmarks of cellular senescence were observed after EZH2 shRNA treated, including flat and enlarged morphology and an increase in SA-β- gal activity but not in AGS cells. |
| Target gene: | H3K27ME3//INK4/ARF//P14//P15INK4B//P16 |
| Official symbol(s): | H3K27ME3//CDKN2A//P14//P15INK4B//P16 |
| R-AG-Target gene: | --//--//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR |
| Target gene description: | Moreover, a significant decrease of H3K27me3 was also observed compared with negative control.Western blot analysis confirmed the activation of INK4/ARF locus leading by EZH2 depletion at protein level.For cells with EZH2 down-regulated, p14ARF, p15INK4b and p16INK4a transcript levels were increased dramatically compared with control cells . |
| Regulatory pathway: | P15-RB |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN2B-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Meanwhile, depletion of EZH2 increased p15INK4b expression dramatically, demonstrating that EZH2 blocks p15INK4b activation and this regulation maybe make great contribution to cellular senescence induced by EZH2 silencing in MNK28 cells. Moreover, the decreased p15INK4b protein expression was associated with increased hyperphosphorylation of its downstream, Rb. |
Annotation:
Loading,please wait...