| Gene name: | GSK3B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Chang |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | As expected, at the subcytotoxic doses of SB415286, cells acquired senescence phenotypes in dose- and time-dependent manners, as shown by the gain of SA-β-gal activity and flattened, enlarged cellular morphology. |
| Target gene: | GS |
| Official symbol(s): | APC |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Cell viability assay//SA-β-gal activity assay |
| Target gene description: | GS phosphorylation was decreased when SB415286, a small molecule inhibitor of GSK3 (Coghlan et al., 2000), was applied to Chang cells at concentrations < 15 g mL 1, but rather increased at higher concentrations of SB415286 (20 and 25 g mL 1).Clearly, GS was activated by dephosphorylation in these conditions, resulting in the progressive accumulation of glycogen along with the senescence phenotypes, such as SA-β gal and induction of p16 and p21. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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