| Gene name: | PIM1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//SAHF//Knockdown |
| Description: | Furthermore, PIM-1 knockdown resulted in a reduced percentage of cells with senescence-associated b-galactosidase (SA-β-gal) staining and SAHF formation, two well-known markers of cellular senescence. |
| Target gene: | HP1γ |
| Official symbol(s): | CBX3 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | IP//Western blot//qPCR |
| Target gene description: | IP with anti-PIM-1 antibody successfully pulled down HP1γ, while reciprocal experiment using anti-HP1γ antibody validated the interaction between PIM-1 and HP1γ in senescent cells. |
| Regulatory pathway: | IL-6-STAT3 |
| R-AG-Pathway: | -- |
| Official symbol(s): | IL-6-STAT3 |
| Pathway experiment: | Luciferase reporter assay//Knockdown |
| Pathway description: | Reporter analysis showed that RasV12 overexpression increased the wild-type PIM-1 promoter activity, and mutation of the STAT3 binding site dramatically attenuated the RasV12- induced promoter activity .In addition, expressing IL-6 shRNA suppresses the PIM-1 promoter activity. |
Annotation:
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