| Gene name: | PTEN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | Western blot//Immunocytochemistry//SA-β-gal activity assay |
| Description: | PTEN depleted MCF7 cells displayed prematurely senescent phenotypes in a p53/p21-dependent manner.The SA-β-Gal positivity of PTEN KO MEFs was dramatically attenuated in Torin1-treated cells compared to Rapa-treated cells. |
| Target gene: | MTORC1//MTORC2//P53 |
| Official symbol(s): | MTORC1//MTORC2//P53 |
| R-AG-Target gene: | --//--//Activation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//IP |
| Target gene description: | The activities of both mTORC1 and mTORC2 were increased along with that of AKT during PICS.Indeed, p53 phosphorylation at S15 was abolished in PTEN-depleted cells expressing mTOR KD. We further conducted an immunoprecipitation (IP) assay with anti-p53, and observed endogenous association of mTOR with p53 during PICS . Raptor and Rictor were also found in the p53 immunoprecipitates, indicating that both mTORC1 and mTORC2 physically associated with p53. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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