| Gene name: | SALL1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7,MDA,E0771 |
| Gene ID: | 6299 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 29625565 |
| Experiment: | SA-β-gal activity assay |
| Description: | We observed that transfection of SALL1 in MCF-7, MDA, and E0771 tumor cells significantly increased the number of SA-βGal+ cells, indicating the induction of tumor cell senescence. |
| Target gene: | ATM//H2AX//53BP1//CHK2 |
| Official symbol(s): | ATM//H2AX//TP53BP1//CHK2 |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Flow cytometry |
| Target gene description: | Overexpression of SALL1 significantly induced Activate, phosphorylated ATM in MCF-7, MDA and E0771 cancer cells. We observed that transfection of SALL1, but not SALL4 or control vector also significantly induced phosphorylation of H2AX, 53BP1 and CHK2 in MCF-7, MDA and E0771 cells (Data not shown). |
| Regulatory pathway: | P38 MAPK//ERK1/2//MTOR |
| R-AG-Pathway: | --//--//Activation |
| Official symbol(s): | MAKP14//MAPK3-MAPK1//MTOR |
| Pathway experiment: | Western blot |
| Pathway description: | We first determined the activation and phosphorylation of MAPKs, including ERK1/2, p38 and JNK in breast cancer cells transfected with SALL1 using western blot analyses. We found that transfection of SALL1 but not mutated SALL1 selectively activated ERK1/2 and p38, but not JNK, resulting in significantly enhanced phosphorylation of ERK1/2 and p38 in both MCF-7 and E0771 breast cancer cells. Transfection of SALL1 but not mutated SALL1 in both MCF-7 and E0771 breast tumor cells significantly induced the phosphorylation of mTOR, p70S6K, and 4E-BP1, further confirming the activation of mTOR signaling in tumor cells after SALL1 expression. |
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