| Gene name: | CAV1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116,NIH-3T3 |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | We found that only the expression of the mutant form of Nrf2 that cannot bind to caveolin-1 and is constitutively Activate (Φ A-Nrf2) protected cells from oxidative stress induced premature senescence, as shown by reduced staining for senescence-associated β-galactosidase activity (SA-β-gal) and p21Waf1/Cip1 protein expression. The stable overexpression of caveolin-1 in HCT116 cells potentiated the oxidative stress–induced up-regulation of p21Waf1/Cip1 and p16 expression and promoted the development of SA-β-gal–positive cells. |
| Regulatory pathway: | NRF2 |
| R-AG-Pathway: | -- |
| Official symbol(s): | NFE2L2 |
| Pathway experiment: | SA-β-gal activity assay//Pull-down assay |
| Pathway description: | We found that only the expression of the mutant form of Nrf2 that cannot bind to caveolin-1 and is constitutively Activate protected cells from oxidative stress induced premature senescence, as shown by reduced staining for senescence-associated β-galactosidase activity (SA-β-gal) and p21Waf1/Cip1 protein expression. We found that WT -Nrf2-His bound to caveolin 1 (residues 82–101)–GST. |
Annotation:
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