| Gene name: | ING1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PCa |
| Gene ID: | 3621 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | HL |
| PMID: | 26993046 |
| Experiment: | SA-β-gal activity assay |
| Description: | By conducting SA-β-Gal staining in the stably transduced cells, ING1b was shown to induce cellular senescence in PCa cells . Further SA-β-Gal assays were performed with different ligand treatments and indicate that ING1b enhances the level of senescent cells without synergizing with AR . |
| Target gene: | P16//P27//AR |
| Official symbol(s): | P16//P27//AR |
| R-AG-Target gene: | Upregulation//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Co-IP//Pull-down assay//Fluorescence microscopy |
| Target gene description: | The results indicated that ING1b upregulates mRNA and protein expression levels of p16 in LNCaP-ING1b cells.Moreover, ING1b has an additive effect along with the AR antagonist Casodex on p16 mRNA induction. ING1b also stabilized p27 protein level in LNCaP-ING1b cells while not altering p27mRNA level (data not shown).In line with the previous results, AR protein was indeed detected in the immunoprecipitates with anti-ING1 antibody whereas no AR was detected in the negative control using a nonspecific antibody.AR protein could be detected with GST-ING1b eluates whereas the negative GST control did not show AR signal. Quantitative colocalization analyses of the images from cotransfected cells suggest that ING1b colocalizes with AR in the nucleus excluding the nucleolus with a high statistical significance. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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