| Gene name: | SERPINE1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | Luciferase reporter assay//SA-β-gal activity assay//Western blot//Knockdown |
| Description: | PAI-1 knockdown resulted in concomitant decrease in extracellular IGFBP3 levels and suppression of senescence induction. These results suggest that doxorubicin-induced DNA damage enhances the secretion of PAI-1, which in turn results in elevated extracellular IGFBP3 and induction of senescence in MCF-7 cells. |
| Target gene: | T-PA//IGFBP3 |
| Official symbol(s): | T-PA//IGFBP3 |
| R-AG-Target gene: | --//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot |
| Target gene description: | The reduction of IGFBP3 levels upon PAI-1 knockdown was alleviated by the addition of anti t-PA antibody to the culture medium, supporting the role for t-PA in mediating IGFBP3 reduction upon PAI-1 knockdown.Recombinant PAI-1 increased extracellular IGFBP3 levels and induced senescence in MCF-7 cells, which was abolished by knockdown of IGFBP3. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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