| Gene name: | CPT1C |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PANC-1 |
| Gene ID: | 126129 |
| Category: | protein coding |
| Phenotype: | Pancreatic cancer |
| Experimental category: | L |
| PMID: | 29317762 |
| Experiment: | BrdU assay//SA-β-gal activity assay//DAPI staining//qPCR |
| Description: | Upon CPT1C loss, the number of PANC-1 cells Activately replicating DNA decreased,consistent with effective inhibition of colony formation in a cell concentrationdependent manner , indicating that PANC-1 cells lacking expression of CPT1C have reduced proliferation. In addition, strong SA-β-gal activity indicated that CPT1C depletion led to the severe Y. Wang et al. senescence state of PANC-1 cells.As expected, loss of CPT1C caused PANC-1 cells to change shape and increase cytoplasmic granularities. DNA SAHF was specifically enriched in CPT1C depleted PANC-1 cells, while controls were excluded from SAHF. CPT1C-depleted PANC-1 cells also had abnormal nuclear size, when compared to control cells.Furthermore, a significant increase in IL8 mRNA levels confirmed a sharp induction of the SA phenotypic changes observed by the SA-β-gal assay. Finally, the TRF length assay revealed that CPT1C depletion inhibited the elongation of telomeres by 0.8 kb in PANC-1 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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