| Gene name: | PPM1B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Description: | In contrast to the basal level of staining observed in the control cells, the PPM1B depleted cell lines developed a characteristic senescence-associated β-GAL staining pattern with a 10-fold increase in the percentage of stained cells. Moreover PPM1B depleted cells displayed a flattened and irregular cell shape with a bigger cell volume which is typical of senescent cell morphology . In addition, the gene expression levels of four senescence markers were examined by RT-qPCR in these cells . The transcript abundance of p21, plasminogen activator inhibitor 1 (PAI), SA-βgalactosidase (β-GAL) and p16 all showed a two to threefold increase in gene expression upon PPM1B depletion compared to those infected with control virus. The increase in transcript abundance is consistent with the immunoblot analysis against of PAI-1, p21 and p16, suggesting that the upregulation of senescence markers occurs at both the transcript and protein levels. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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