| Gene name: | SMARCA4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SW48 |
| Gene ID: | 6597 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 28182012 |
| Experiment: | Western blot//qRT-PCR//SA-β-gal activity assay//CCK-8 assay//Flow cytometry//DAPI staining |
| Description: | Data showed that the percentages of SA-β-galpositive cells in SW48 sh-BRG1 cells significantly increased compared with sh-Con cells in both DOX treatment and nontreatment conditions .The results showed that the growth rates significantly decreased in BRG1 KD groups, suggesting that BRG1 KD inhibited cell proliferation. Cell cycle analysis by flow cytometry detection was performed, and the results revealed that BRG1 KD led to cell cycle arrest at G2-M phase. Furthermore, we examined SAHF by staining 4,6-diamidino-2-phenylindole (DAPI), which showed distinct SAHF formation in BRG1 KD cells as observed by laser confocal fluorescence microscopy scanning. |
| Regulatory pathway: | SIRT1-P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | SIRT1-TP53-CDKN1A |
| Pathway experiment: | Western blot//qRT-PCR//IHC//SA-β-gal activity assay//Co-IP//Immunofluorescence |
| Pathway description: | Our data showed that the increase of p21 induced by BRG1 KD was abrogated by the treatment of p53 siRNA. Moreover, SA-β-gal-positive cells stopped increasing after the KD of BRG1 with p53 siRNA in both DOX treatment and non-treatment conditions.The regulation of BRG1 in the p53/p21 pathway was further confirmed by using another shRNA against BRG1. These results suggested that KD of BRG1 promotes senescence by activating the p53 pathway in CRC cells. |
Annotation:
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