| Gene name: | PON1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | To evaluate the influence of PON1 knockdown on ageing of HDMECs, we measured cellular senescence by SA-β-gal staining. Cell senescent morphology, such as an enlarged and flattened shape with an increased diameter, was observed in aged HDMECs at passage 2 and in PON1 siRNA #2-treated HDMECs at 48 h post-transfection. The number of cells positive for SA-β-gal staining increased significantly compared to early passage HDMECs at passage 8 and in scrambled siRNA-treated HDMECs. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | In Western blot, the expression of p16 in PON1 knockdown HDMECs was observed. At 48 h post-transfection, the protein expression of p16 in PON1 siRNA-treated HDMECs was significantly higher than the expression level in scrambled siRNA-treated HDMECs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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