| Gene name: | CSNK2A1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Experiment: | SA-β-gal activity assay |
| Description: | To detect senescence, p53-/- and wild-type HCT116 cells were treated with CKII inhibitors, apigenin and DRB, and then stained for SA-β-gal activity. In the p53 positive HCT115 cells, there was a significant dosedependent increase in SA-β-gal activity in response to apigenin and DRB. |
| Target gene: | P52//RB |
| Official symbol(s): | P52//RB |
| R-AG-Target gene: | --//-- |
| Subcategory: | Phosphorylation |
| Target gene experiment: | SA-β-gal activity assay//Western blot |
| Target gene description: | However, p53 negative HCT116 cells showed only slight signs of senescence demonstrating that p53 is required for the induction of senescence through CKII inhibition.The level of hyperphosphorylated RB protein decreased while hypophosphorylated form increased in wildtype HCT115 cells treated with CKII inhibitor. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | SA-β-gal activity assay//Western blot//RT-PCR |
| Pathway description: | When these transfectants were stained for SA-β-gal activity, p53 negative HCT116 cells exhibited an apparently lower rate of SA-β-gal activity compared to p53 positive HCT116 cells. In p21Cip1/WAF1 positive HCT116 cells, there was a significant increase in SA-β-gal activity in response to apigenin and DRB. However, p21Cip1/WAF1 negative HCT116 cells showed only slight signs of senescence demonstrating that p21Cip1/WAF1 is required for the induction of senescence via CKII inhibition. Cells treated with apigenin showed an increase both in the p21Cip1/WAF1 protein and mRNA levels, suggesting that CKII inhibition up-regulates p21Cip1/WAF1 expression at the transcriptional level . |
Annotation:
Loading,please wait...