Gene name: | FOXO3 |
Aging type: | Prevent |
Aging characteristic: |
Tissue type: | -- |
Cell name: | HDF |
Experiment: | SA-β-gal activity assay//Cell morphological analysis//Western blot//Knockdown |
Description: | Senescence Phenotypes in FOXO3a-siRNA Cells Senescent cells are resistant to mitogen-induced proliferation , express senescence-associated β-gal, and have a characteri stically enlarged, flattened morphology. We measured cell growth and senescence-associated β-gal activity in FOXO3a-siRNA cells. FOXO3a-siRNA cells had an enlarged, flattened cell morphology, like old cells. The percentage of blue cells (indicating senescence-associated β-gal activity) was higher in FOXO3a-siRNA cells than in vectortransfected cells.As expected, the levels of p53 and p21 proteins were up-regulated in FOXO3a-siRNA cells as well as in old cells . |
Regulatory pathway: | -- |
R-AG-Pathway: | -- |
Pathway experiment: | -- |
Pathway description: | -- |
Annotation:
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