| Gene name: | MAGEA2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay |
| Description: | Analysis of senescenceassociated β-galactosidase activity (SA-β-Gal), a well-known marker of senescence,demonstrated that MageA2 expression significantly reduced SA-β-Gal levels in PMLIV-expressing cells. |
| Target gene: | PMLIV |
| Official symbol(s): | PML |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Expression of MageA2 together with PMLIV/p300 strongly decreased PMLIV acetylation. Importantly, siRNA-mediated downregulation of MageA2 in U2OS cells stably expressing PMLIV resulted in increased levels of sumoylated PMLIV, demonstrating the involvement of endogenous MageA2 in the regulation of PMLIV sumoylation. |
| Regulatory pathway: | PML-P53 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | PML-TP53 |
| Pathway experiment: | Cell morphological analysis//qRT-PCR//SA-β-gal activity assay//BrdU assay//Western blot |
| Pathway description: | To this end, normal human fibroblasts were co-transduced using retroviral vectors expressing PMLIV with an empty vector or in combination with MageA2 or alternatively MageA4. After 10 days under selective culture conditions, PMLIV transduced cells showed all the features of the senescence process, because they ceased to proliferate at sub-confluent densities and became flat and enlarged. Importantly, cells co-expressing PMLIV and MageA2 did not show major morphological changes and behaved similarly to control cells. Colocalization between MageA2 and PMLIV was observed in these cells (data not shown). Analysis of senescenceassociated β-galactosidase activity (SA-β-Gal), a well-known marker of senescence, demonstrated that MageA2 expression significantly reduced SA-β-Gal levels in PMLIV-expressing cells. Moreover, cells expressing MageA2 and PMLIV showed higher levels of BrdU incorporation with respect to those expressing only PMLIV. Cells overexpressing MageA2 alone behaved indistinguishably from control or MageA4 transduced cells. Importantly, MageA2 expression correlated with reduced p21 protein levels, probably due to an effect of MageA2 on efficient p53 activation.Moreover, MageA2 expression affected p53 activity in this cellular system, as assessed by quantification of p53 target genes, thus confirming that MageA2 downregulates the p53-dependent response. |
Annotation:
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