| Gene name: | SUV39H1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WI-38 |
| Experiment: | SA-β-gal activity assay//Cell viability assay//Western blot |
| Description: | In order to test this, we treated cells at different PD levels with chaetocin, a specific inhibitor of SUV39H1 .First, we tested the cytotoxicity of increasing concentrations of chaetocin in the cells for all three PD levels, and found that cell cultures of all senescence states maintained a high level of viability when treated with 5 or 1 nM chaetocin and decreased rapidly at higher concentrations . Interestingly, the PD 54 cells, which express lower levels of SUV39H1, were more tolerant to higher concentrations of chaetocin, as seen by their viability at 48 h and 96 h following chaetocin treatment. In addition to a decrease in viability, increasing concentrations of chaetocin induced greater senescence in PD 47 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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