| Gene name: | EZH2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Gene ID: | 2146 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 27882937 |
| Experiment: | SA-β-gal activity assay |
| Description: | Consistently, LDMcaused senescence was reduced by overexpressed EZH2 level. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//SA-β-gal activity assay |
| Target gene description: | We then checked whether EZH2 knockdown may affect p21 expression, as well as senescent phenotype. Three siRNAs caused apparent reduction of EZH2 expression in both cells, and all the siRNAs significantly increased p21 expression in SW620 cells, whereas two of them (2# and 3#) induced marked p21 expression in HCT116 cells. Although si-EZH2 (1#) failed to increase p21 expression in HCT116 cells for unknown reason, this result confirms that EZH2 negatively regulated p21 expression. Furthermore, EZH2 knockdown with siRNA (3#) caused senescent phenotype, indicating that EZH2 may mediate LDM-induced senescence as well. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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