| Gene name: | KCNH7 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | A375 |
| Experiment: | Western blot//Flow cytometry |
| Description: | We found that, in agreement with our previous investigation, stimulation of Kv11.3 strongly upregulated both senescent markers in melanoma cells. However, in contrast with the effect of NS1643 in breast cancer cells in which we detected an arrest of the cell cycle in G0/G1 phase, analyses of the cycle progression in A375 melanoma shows that stimulation of Kv11.3 channel significantly increased the population of cells in the G2/M phase compared to untreated cells suggesting that NS1643 determined an arrest of the cell cycle in the G2/M phase of the cell cycle. |
| Regulatory pathway: | RAF-MEK-ERK |
| R-AG-Pathway: | -- |
| Official symbol(s): | ZHX2-MAP2K7-MAPK1 |
| Pathway experiment: | Western blot |
| Pathway description: | Interestingly, we found that concomitant to the effect of NS1643 on cell cycle proteins, NS1643 strongly inhibited both MEK and ERK activities as indicated by the decreased phosphorylation of these proteins. |
Annotation:
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