| Gene name: | UBE2E3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | RPE-1 |
| Experiment: | ELISA//SA-β-gal activity assay//EdU assay//Western blot//Flow cytometry |
| Description: | Depletion of UBE2E3 led to a loss of proliferation, as indicated by a failure of siUBE2E3 cells to incorporate 5- ethynyl-2 -deoxyuridine (Edu), a marker of newly-synthesized DNA ,an increase in cell size,a robust expression of senescence-associated β-galactosidase , a decrease in intracellular HMGB1 content,redistribution of lamin B from its primary localization at the nuclear envelope to a punctate, nucleoplasmic distribution and a concomitant decrease in lamin B1 mRNA expression,increased transcription of the tumor suppressor p16INK4a, and increased secretion of the pro-inflammatory cytokine IL-6 . |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | SA-β-gal activity assay//EdU assay |
| Pathway description: | Using a combination of co-knockdowns and measuring both SA-β-Gal expression as well as Edu incorporation in the same cells, 4 days after siRNA administration, we found that silencing the expression of either p53 or p21CIP1/WAF1 mitigated the siUBE2E3- induced SA-β-gal expression whereas silencing either p21CIP1/WAF1 or p16INK4a mitigated the cell cycle exit. |
Annotation:
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