Aging Overview

Aging gene

Gene name: EZH2
Aging type: Prevent
Aging characteristic:
Tissue type: --
Cell name: SK-Mel-119,UCD-Mel-N,SK-Mel-173
Gene ID: 2146
Category: protein coding
Phenotype: Melanocytic nevi
Experimental category: L
PMID: 21383005
Experiment: Immunofluorescence//Flow cytometry//SA-β-gal activity assay//qRT-PCR//SAHF//Cell morphological analysis//Knockdown
Description: Growth of EZH2 siRNA cells was significantly decreased compared to control-transfected cells. EZH2 siRNA cells displayed a flattened, enlarged morphology and expressed SA-β-Gal. In addition to SA-β-Gal expression, additional senescence markers following extended EZH2 depletion (6 days) include SAHFs and the heterochromatin-associated H3K9me3 modification as well as an increase in cell size, as evaluated by tubulin immunofluorescence. Flow cytometric analysis demonstrated that the proportion of cells in the S phase decreased in EZH2 siRNA cells with a concomitant increase of cells in the G1 phase, a significant difference in all 3 cell lines tested. In SK-Mel-173 cells, we also noticed a slight increase in the proportion of cells in the G2 phase.


Regulatory relationship

Target gene: P21
Official symbol(s): P21
R-AG-Target gene: Inhibition
Subcategory: Unclear
Target gene experiment: Western blot
Target gene description: However,depletion of EZH2 elevated expression of p21 in 5/6 cell lines.

Regulatory pathway: --
R-AG-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



About risk SNP and eQTL

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