| Gene name: | SLC25A5 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U2OS,MCF-7 |
| Experiment: | BrdU assay//Knockdown//Cell morphological analysis//SA-β-gal activity assay |
| Description: | Down-regulation of ANT2 by siRNA led to a decreased BrdU incorporation indicating inhibition of cell proliferation .Further decrease in BrdU incorporation was observed after the combined treatment. Although short term knock-down of ANT2 did not enhance senescence associated β-galactosidase staining, enhanced vacuolization, typical of senescent cells, was observed in cells with combined treatment. |
| Target gene: | NF1/SMAD |
| Official symbol(s): | NF1/SMAD |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | IP//CHIP//qRT-PCR//Flow cytometry |
| Target gene description: | Induction of the NF1/Smad complexes and binding of NF1 to the ANT2 NF1-dependent repressor elements followed the same time course as ANT2 repression in U2OS cells. |
| Regulatory pathway: | TGFβ |
| R-AG-Pathway: | -- |
| Official symbol(s): | TGFB1 |
| Pathway experiment: | qRT-PCR//Flow cytometry |
| Pathway description: | These events are also closely correlated with the expression and secretion of TGF-β suggesting that formation of the NF1/Smad complexes is initiated through TGF-β signaling. |
Annotation:
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