| Gene name: | PRKAA1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Lung |
| Cell name: | BEAS-2B |
| Gene ID: | 5562 |
| Category: | protein coding |
| Phenotype: | Chronic obstructive pulmonary disease |
| Experimental category: | L |
| PMID: | 28186975 |
| Experiment: | qRT-PCR//Cytokine assay//Immunocytochemistry//Western blot |
| Description: | Transfection of AMPKα1/α2 siRNA increased the mRNA of p16, p21 and p66shc, but reduced klotho gene expression in BEAS-2B cells.We also determine the expression of p16 and p21, markers of cellular senescence, in lung tissues using Western blot and immunohistochemistry. |
| Target gene: | SOD2//SIRT3 |
| Official symbol(s): | SOD2//SIRT3 |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Immunocytochemistry//Western blot |
| Target gene description: | In response to elastase, the abundance of SOD2 and SIRT3 proteins was significantly reduced as compared to saline control.The expression of SOD2 and SIRT3 proteins was augmented by prophylactic treatment of metformin, whereas Compound C further caused reduction of SOD2 and SIRT3 proteins in emphysematous lungs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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