| Gene name: | CREG1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Fibroblast |
| Gene ID: | 8804 |
| Category: | protein coding |
| Phenotype: | Li-Fraumeni Syndrome |
| Experimental category: | HL |
| PMID: | 21263217 |
| Experiment: | Western blot//MTT assay//Cell proliferation assay |
| Description: | The expression level of CREG1 fits the criteria of senescence-associated gene, decreased during immortalization and increased in replicative and 5-aza-dC-induced senescence justifying further investigation of the role of CREG1 in cellular senescence.We found that CREG1 overexpression decreased cell growth in immortal LFS cells when compared with pIRES empty vector transfected cells demonstrated by both MTT and cell counting proliferation assays. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Cell cycle analysis//SA-β-gal activity assay//Western blot//Cell morphological analysis |
| Target gene description: | Cell cycle analysis showed an increase in the G0-G1 cell population and a decrease of S-phase population in cells cotransfected with CREG1 and p16INK4a compared to cells transfected with either CREG1 or p16INK4a alone.In p16-transfected cells and more so in cells cotransfected with CREG1 and p16INK4a, we observed cell characteristics indicative of senescence, a large and flat morphology with an increase of SA-β-galactosidase staining.We found that cells coexpressing CREG1 and p16INK4a showed a marked decline in protein levels of cyclin A and B . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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