| Gene name: | KIF2C |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF,HUVEC |
| Experiment: | SA-β-gal activity assay//Western blot//RT-PCR |
| Description: | We tried to investigate the role of MCAK/Kif2C in cellular senescence.Cellular senescence of HDFs and HUVECs was confirmed by increases in SA-β-gal activity and p53 levels .Decreased expression levels of MCAK/Kif2C were shown in old cells by RT-PCR, real-time PCR, and Western blotting. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//RT-PCR//Western blot |
| Pathway description: | Knockdown of p53, p16, and MCAK/Kif2C were confirmed by RT-PCR and Western blotting. A decrease in cell proliferation and an increase in SA-β-gal activity by MCAK/Kif2C knockdown were not observed in p53 siRNA cells, but were evident in p16 siRNA cells. Senescent-like cell morphology was also shown in p16 siRNA cells, but not p53 siRNA cells . These results support the view that premature cellular senescence induced by MCAK/Kif2C knockdown might be regulated via a p53-dependent pathway. |
Annotation:
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