| Gene name: | WWP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry |
| Description: | Senescence markers for WWP1-overexpressing and WWP1 shRNA-infected cells were then monitored at the same time points as their corresponding control cells. The WWP1 shRNA-transfected 2BS cells displayed enlargement, flattening, and the accumulation of vacuolated cytoplasmic inclusions. However, no significant morphological changes were observed in the WWP1-transfected cells, which were similar to young 2BS cells. Most of the WWP1 shRNA-transfected cells showed strong blue SA-β-gal staining similar to senescent cells . In contrast, only scattered SA-β-gal-positive cells were found in WWP1-transfected cells compared with the corresponding control cells. 2BS cells overexpressing WWP1 showed increased S and reduced G1 compartments. It was obvious that WWP1 shRNA-infected 2BS cells entered G1cell cycle arrest. |
| Regulatory pathway: | P27 |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN1B |
| Pathway experiment: | Knockdown//RT-PCR//Western blot |
| Pathway description: | The protein levels of p27Kip1were markedly depressed in WWP1-transfected HeLa cells as compared with the vector-transfected cells, whereas the protein levels of p16INK4a and PTEN were invariable. In addition, we silenced WWP1 in HeLa cells. Similarly, there was no detectable change in the protein levels of p16INK4a or PTEN, but a marked increase in the p27Kip1 protein level was found. Moreover, the RT-PCR analysis demonstrated no alteration in p27Kip1mRNA levels in both WWP1-overexpressing and knockdown cells, which indicated that WWP1 might normally decrease the p27Kip1 protein level but not the mRNA level. Similar results were observed in 2BS cells. |
Annotation:
Loading,please wait...