| Gene name: | SMARCB1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Lac-hSNF5 |
| Gene ID: | 6598 |
| Category: | protein coding |
| Phenotype: | Malignant Rhabdoid Tumor |
| Experimental category: | L |
| PMID: | 14604992 |
| Experiment: | BrdU assay//DAPI staining//SA-β-gal activity assay//Flow cytometry |
| Description: | We found that hSNF5 induction dramatically impaired cell proliferation, even when cultured in the presence of 1 % serum . In contrast, addition of IPTG had no effect on Lac-empty control cells lacking the hSNF5 gene. Similar results were obtained with independently isolated Lac-hSNF5 cell lines (data not shown). We also assessed the effect of hSNF5 on the colony-forming capacity of Lac-hSNF5 cells, and found that hSNF5 expression dramatically reduced the number of colonies formed, after seeding these cells at a very low density.We next determined which stage of the cell cycle is affected by hSNF5.Expression of hSNF5 leads to impaired DNA synthesis, indicated by the strongly reduced incorporation of BrdUrd. FACS analysis revealed that the hSNF5-expressing cells accumulate in G1, suggesting that the cell cycle block affects entry into S-phase. |
| Regulatory pathway: | P16-PRB |
| R-AG-Pathway: | -- |
| Official symbol(s): | CDKN2A-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | Western immunoblotting revealed a strong up-regulation of p16INK4aprotein levels following hSNF5 expression, whereas no increase or even a small reduction of p14ARFwas observe.Because p16INK4a is a specific inhibitor of the Rb-regulating cyclin D1-CDK4 complex, we determined the phosphorylation status of pRb in Lac-hSNF5 cells. In support of a role of hSNF5. |
Annotation:
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