| Gene name: | HJURP |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF,HUVEC |
| Experiment: | SA-β-gal activity assay//DAPI staining//Flow cytometry//RT-PCR//Western blot//Cell counting |
| Description: | Ectopic expression of HJURP in senescent cells increased cell proliferation in a time-dependent manner and resulted in morphological changes similar to young cells and a decrease in SA-β-gal staining activity.In addition, the upregulation of HJURP decreased the cell population in the G1 phase of the cell cycle, which is increased in old cells and cells with control vector and elevated the G2/M cell populations, which is declined in old cells and control vector. Because upregulation of HJURP levels partially reversed cellular senescence, we examined the effects of HJURP knockdown on senescence phenotypes in young HDFs and HUVECs by transfection of HJURP siRNAs. HJURP knockdown was confirmed by RT-PCR and Western blotting . Although the levels of pA TM, p53, and p21 were elevated, the levels of pRb and CENP-A were repressed by HJURP knockdown. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//Western blot//RT-PCR//SA-β-gal activity assay |
| Pathway description: | To determine the pathway involved in the regulation of cellular senescence by HJURP reduction, we knocked down p53 or p16 levels in young cells and measured the effects of HJURP downregulation on cellular senescence. The levels of HJURP, p53, and p16 were confirmed by RT-PCR and Western blotting. HJURP knockdown decreased cell proliferation in p16 siRNA cells but not in p53 siRNA cells. Similarly, depletion of HJURP increased SA-β-gal staining activity in p16 siRNA cells but not in p53 siRNA cells. |
Annotation:
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