| Gene name: | AGR2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LNCaP,DU 145,PC-3 |
| Experiment: | MTT assay//qPCR//Western blot//Flow cytometry//SA-β-gal activity assay |
| Description: | Forced expression of AGR2 in LNCaP or DU145 cells for 72 h resulted in a marginal increase in the cell viability as measured by MTT assays. V alidation of the AGR2 expressions at mRNA and protein levels was performed by quantitative PCR and western blot assays. AGR2 expressions were markedly increased in the transfected LNCaP or DU145 cells compared with the vector-transfected cells. Reduced endogenous AGR2 in PC-3 cells led to decreased cell viability, whereas no decreases were observed in cells transfected by control oligonucleotides.Transfection of LNCaP, PC-3 and DU145 cells with AGR2i led to significantly increase in the percentage of cells in G /G1phase compared with the control cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...