| Gene name: | TFAP4 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | RPE |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis |
| Description: | The morphological changes in AP4-expressing cells, including enlarged nuclei and flattened cytoplasm, were characteristic of cellular senescence .When AP4 expression was induced by adding DOX to the media, the proliferation profiles of RPE-AP4 cells started to differ on Day 12 ARC from the cells without receiving DOX. AP4-expressing cells stopped proliferating, and the status of these cells remained constant for at least additional 12 days.To quantify the extent of AP4-induced senescence, the number of blue SA-β-gal-positive cells was counted.The results showed that substantially more AP4-expressing cells than control cells were SA-β-gal positive. |
| Regulatory pathway: | P14-P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | CDKN2A-TP53 |
| Pathway experiment: | qRT-PCR//Immunofluorescence |
| Pathway description: | Immunofluorescence microscopy was used to investigate the expression of p14ARF, p53,and Rb proteins in AP4-induced senescent RPE cells. p53 protein was expressed primarily in the large senescent AP4-expressing cells but not in DN-AP4-expressing cells.A similar expression pattern was observed in p14ARF, which appeared as small speckles distributed throughout the nucleoplasm but not the nucleolus of AP4-expressing cells .To determine whether AP4 can regulate p53 at the transcriptional level, the levels of p53 mRNA were quantified using qRT-PCR. The p53 gene has two promoters, P1 and P2 ,which are the transcription starting sites of the p53 gene and produce transcripts of different lengths. |
Annotation:
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