| Gene name: | MCAM |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | CD1462,CD146+ |
| Experiment: | SA-β-gal activity assay//qRT-PCR//TRAP assay//Immunoblotting//Knockdown |
| Description: | Specifically,the growth of CD1462cells ceased at P12, whereas CD146+ cells continued to grow until P15. In qPCR analysis, CD146+ cells showed high expression levels of stemness genes compared with those observed in CD1462 cells . Similarly, telomerase activities of CD146+ cells were significantly higher than those of CD1462 cells . Western blot analysis indicated that CD1462 cells showed enhanced expression of pho-p53 and p16 relative to that observed in CD146+ cells, as well as significantly augmented SA-β-gal activity and cell areas .Cells with silenced CD146 expression exhibited a reduced duration of proliferation during the culture period and lower growth rates, compared with the respective measures made in na?ve cells or scrambled siRNA-transfected cells. SA-β-gal activities and cell areas were significantly increased in CD146 siRNA transfectants. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...