| Gene name: | PIR |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | WM266-4,IGR-37 |
| Experiment: | Knockdown//Western blot//Colony formation assay//SA-β-gal activity assay//Cell morphological analysis |
| Description: | The growth rate was significantly reduced in both mela-noma cell lines stably expressing shPIR constructs,compared with that of cells bearing the empty pSICO-R. A colony formation assay was also performed to measure the growth rate at low density. The number of colonies formed by shPIR cells was significantly reduced in both cell lines.The shPIR-transduced cell lines exhibited changes in size and morphology compatible with cellular senescence (flattened and enlarged phenotype). We therefore performed a senescence-associated β galactosidase(SA-β-galactosidase) assay and found that PIR knockdown in WM266-4 cells resulted in an increase from 2.1% positivity in control cells to 41.3%, 16.6%, and 37.1% in shPIR#7, #16, and #19 transduced cells, respectively. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...