| Gene name: | IGF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | VSMC |
| Experiment: | SA-β-gal activity assay |
| Description: | We observed a strong induction of SA-β-gal activity following IGF-I stimulation in the confluent cells |
| Regulatory pathway: | ROS-P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | ROS1-TP53 |
| Pathway experiment: | Immunoblotting//SA-β-gal activity assay |
| Pathway description: | We examined the involvement of ROS in the induction of cellular senescence by IGF-I.Treatment with an ROS scavenger, N-ace-tylcysteine (NAC), significantly suppressed the IGF-I-induced increase in SA-β-gal activity. Furthermore, NAC treatment also suppressed the induction of cH2AX, p53, and p21 proteins, indicating that ROS are involved in IGF-I-induced cellular senescence. Treatment with an alternative ROS scavenger,Trion, also produced similar results (data not shown). In addition,we used p53-/- MEFs to clarify the role of p53 in IGF-I-induced cellular senescence; in the absence of p53, IGF-I-induced SA-β-gal activation and p21 augmentation were inhibited. |
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