| Gene name: | TIMELESS |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | Western blot//qRT-PCR |
| Description: | The expression of circadian protein TIMELESS was down-regulated during the onset of OIS, while other members of circa-dian clock showed no significant changes in their expression.The downregulation of TIMELESS was also confirmed inreplicative senescence and H2O2 induced senescence,suggesting TIMELESS downregulation might be a commonevent happened during cellular senescence. |
| Target gene: | E2F1 |
| Official symbol(s): | E2F1 |
| R-AG-Target gene: | -- |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Knockdown//Luciferase reporter assay//CHIP//qRT-PCR//Western blot |
| Target gene description: | Chromatin immunoprecipitation (ChIP) assay was utilized to confirm the binding of E2F1 to the endogenous TIMELESS promoter.Interaction between E2F1 and TIMELESS promoter region was detected by qRT-PCR.As expected, interaction between E2F1 and TIMELESS promoter was observed in young 2BS cells.Furthermore, the mutation in E2F1 binding site of TIMELESS promoter region significantly reduced the luciferase activity in young 2BS cells.Knockdown of E2F1 by a sh-E2F1 lentiviral vector significantly down-regulated TIMELESS mRNA level in young 2BS cells.All above data support that E2F1 could bind to TIMELESS promoter and regulated the expression in young 2BS cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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