| Gene name: | SIRT6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | 2BS |
| Experiment: | Western blot//RT-PCR//Colony formation?assay//Flow cytometry |
| Description: | Western blot analysis revealed that the expression of SIRT6 was high in young cells, but decreased significantly during cellular senescence. Consistently, RT-PCR analysis revealed that mRNA levels of SIRT6 decreased in senescent cells .Another hallmarker of senescent cells is the ability of colony formation. We observed that 2BS cells overexpressing SIRT6 formed more colonies than control cells. However, SIRT6 shRNA-infected cells showed less colonies compared to control cells .It was obvious that SIRT6 shRNA-infected 2BS cells entered G1 cell cycle arrest. |
| Target gene: | P27 |
| Official symbol(s): | P27 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//RT-PCR//SA-β-gal activity assay//Co-IP//Pull-down assay |
| Target gene description: | In addition,SIRT6 silencing did not alter the protein levels of p16, p21, p53 and PTEN, but markedly increased p27 protein levels. Moreover, the real-time PCR analysis demonstrated similar p27 mRNA levels in both SIRT6-overexpressing and knockdown cells, suggesting that SIRT6 might decrease the p27 protein levels but not the mRNA levels.2BS cells co-expressing pLPC,pBabe-p27 exhibited G1 arrest and higher SA-β-gal ctivity. Concurrently, the senescent markers of SIRT6-p27 appeared to be comparable with that of pLPC-pBabe control cells.Thus,the decrease of p27 mediated by SIRT6 is required for the restriction of cellular senescence. Colocalization experiments showed that both SIRT6 and p27 mainly colocalized in the nucleus. To further confirm the interaction of p27 and SIRT6, we performed a GST pull down assay using in vitro transcribed and translated p27 or SIRT6 and purified GST-SIRT6 or GST-p27.SIRT6 displayed a strong association with p27 in vitro. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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