| Gene name: | FXR1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | UMSCC74A,UMSCC74B |
| Gene ID: | 8087 |
| Category: | protein coding |
| Phenotype: | Oral cancer |
| Experimental category: | HL |
| PMID: | 27606879 |
| Experiment: | Knockdown//Flow cytometry//SA-β-Gal activity assay |
| Description: | To investigate the underlying mechanism of reduction in number of viable cells following FXR1 KD, we performed cell cycle analysis of the FXR1 KD and control cells by flow cytometry. FXR1 KD induces cell cycle arrest in G /G1 providing evidence that FXR1 might regulate cell division in oral cancer.Silencing of FXR1 in multiple oral cancer cells also promotes senescence by positive SA-β-gal staining. |
| Target gene: | TERC |
| Official symbol(s): | TERC |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay |
| Target gene description: | When full-length and truncated TERC (28 base deletion at 5’-end,TERCmut） are used for luciferase assay,TERC exhibits increased luciferase activity in control cells compared to TERCmut. However, in FXR1 KD cells, TERC exhibits a decreased luciferase activity compared to TERCmut indicating that FXR1 binds to specific G-rich sequences in these RNAs and possibly controls their turnover.Silencing of FXR1 reduces the level of TERC and subsequently interferes with the telomerase activity. |
| Regulatory pathway: | P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53-CDKN1A |
| Pathway experiment: | RT-PCR//Western Blot |
| Pathway description: | Upregulation ofp53 and p21 protein levels are observed in the absence of FXR1 in WT p53 cell line.RT-PCR、Western Blot：overexpressed FXR1 in HNSCC destabilizes p21 mRNA and reduces its protein expression.concurrent overexpression of p21 and silencing of TERC significantly promoted senescence evidenced by staining of SA-β-gal. |
Annotation:
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