| Gene name: | ASPH |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | FOCUS,HuH-7,HepG2 |
| Gene ID: | 444 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | L |
| PMID: | 26683595 |
| Experiment: | Knockdown//SA-β-Gal activity assay |
| Description: | Knockdown or knockout of ASPH, caused substantial increase in β-gal staining as a marker for the cells undergoing senescence. |
| Target gene: | GSK3β |
| Official symbol(s): | GSK3B |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//IP//Immunoblotting |
| Target gene description: | We found a significant increase of phospho-GSK3β(inactivated form) with equal protein expression of GSK3β when comparing the shASPH to the shLuc control.Direct protein-protein interaction between GSK3βand ASPH was demonstrated by co-immunoprecipitation (IP).Immunoblotting with anti-GSK3β revealed that GSK3β bound to ASPH following co-transfection with WT-GSK3βand ASPH. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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