| Gene name: | TRIB2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | LoVo |
| Gene ID: | 28951 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | L |
| PMID: | 30541550 |
| Experiment: | Knockdown//Flow cytometry//SA-β-gal activity assay//Cell morphological analysis |
| Description: | TRIB2 knockdown cells showed higher rates of flat and enlarged senescence-like morphology.Cell cycle distribution of the cells transfected with TRIB2-specific siRNA were determined by flow cytometry.TRIB2 knockdown dramatically increased the G0/G1-phase ratios and reduced the S-phase ratios. The level of SA-β-gal (a biomarker of senescence) was measured, and data showed that the percentage and strength of SA-β-gal-positive cells were significantly increased in TRIB2 knockdown condition. |
| Regulatory pathway: | AP4-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | REPIN1-CDKN1A |
| Pathway experiment: | Western blot//RT-PCR//Luciferase reporter assay//IP//CHIP |
| Pathway description: | Silencing TRIB2 dramatically up-regulated p21 expression, inhibited cell growth, induced cell cycle arrest and enhanced cellular senescence. The luciferase assay indicated TRIB2 inhibited p21 promoter activities in an AP4-dependent manner. We then performed CCK8 and SA-β-gal staining analysis to examine the effects of AP4 on TRIB2-mediated functions, and found that silencing AP4 could significantly block TRIB2 overexpression-promoted SW48 and LoVo cells growth and enhance TRIB2 overexpression-inhibited cellular senescence. We carried out co-IP assay using SW48 cells or HEK 293T cells co-transfected with Flag-AP4 and His-TRIB2. AP4 and TRIB2 were found to precipitate with each other.The results showed that overexpression of AP4 suppressed and co-transfection of TRIB2 further suppressed the activities of p21 promoter , which indicated TRIB2 enhanced the function of AP4. |
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