| Gene name: | CAV1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MEF |
| Experiment: | SA-β-gal activity assay//Western blot |
| Description: | In contrast, wild type MEFs over-expressing caveolin-1 developed a senescent phenotype 4 days after oxidative stress, as determined by senescent-associated β-galactosidase staining and p21 upregulation. |
| Target gene: | SIRT1 |
| Official symbol(s): | SIRT1 |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Pull-down assay |
| Target gene description: | To determine whether Sirt1 binds directly to caveolin-1, we performed GST pulldown assays using recombinant Sirt1. We found that human recombinant Sirt1 bound to caveolin 1 (residues 82–101)-GST.The incubation with the purified GST-Cav-1 (82–101) peptide inhibited Sirt1 activity by- 45% compared to GST alone. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Pull-down assay//IP//Western blot |
| Pathway description: | We found that human recombinant Sirt1 bound to caveolin 1. However, Sirt1 strongly interacted with caveolin-1 72 hours after stimulation of WI-38 cells with sublethal doses of hydrogen peroxide that we have previously shown induce premature senescence.In contrast, free radicalinduced acetylation of p53 was significantly inhibited in cells lacking caveolin-1. Interestingly, downregulation of Sirt1 by siRNA enhanced oxidant-induced acetylation of p53 in caveolin-1 null MEFs to levels seen in wild type MEFs. |
Annotation:
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