| Gene name: | TWIST |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | SW48 |
| Gene ID: | 100303576 |
| Category: | protein coding |
| Phenotype: | Aging |
| Experimental category: | L |
| PMID: | 17690110 |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | Higher percentage of SA-β-gal-positive cells was found in the Sh-TWIST transfectants (filled columns) compared with the controls (open columns) in response to both agents in a dose-dependent manner. This was associated with a decreased cell proliferation rate examined by BrdUrd incorporating assay.These results suggest that suppression of TWIST expression promotes cellular senescence in PrEC. |
| Target gene: | P14//CH2AX |
| Official symbol(s): | P14//CH2AX |
| R-AG-Target gene: | Downregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | We also found that the p14ARF expression levels were much lower in the TWIST over-expressing cells, either before or after exposure to H2O2 and CP (right panels), compared with the vector control, confirming the negative effect of TWIST on p14ARF. In addition, the p53 and p21 levels were lower in the TWIST over-expressing cells compared with the vector control, especially after drug treatment. Furthermore, we found that the level of cH2AX was higher in the TWIST over-expressing cells either before or after drug treatment compared with the vector control, which was associated with lower levels of phosphorylated Chk1/2 proteins, especially after drug treatment. |
| Regulatory pathway: | MDM2-P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | MDM2-TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | Western blotting analysis showed that the expression levels of p14ARF were much higher in the Sh-TWIST transfectants after exposure to H2O2 or CP in a dose-dependent manner. In contrast, the level of MDM2 was decreased, which was associated with up-regulation of p53 and p21, suggesting that inactivation of TWIST promotes p14ARFMDM2-p53-mediated DNA damage response pathway. |
Annotation:
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